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Transposon insertion upstream of a putative sodium/sulphate symporter is associated with hypervirulence in the soft rot bacterium, Pectobacterium carotovorum
Author(s) -
Kersey Caleb M.,
Heintzman Darren,
Dumenyo C. Korsi
Publication year - 2018
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12694
Subject(s) - biology , pectate lyase , pectobacterium carotovorum , symporter , microbiology and biotechnology , transposable element , mutant , virulence , pectinase , bacteria , biochemistry , gene , enzyme , pathogen , genetics , transporter
Pathogenesis by Pectobacterium carotovorum and the other soft rot bacteria results mostly from the synthesis and secretion of a plethora of exoproteins including plant cell wall‐degrading enzymes and type III effectors. A mini‐Tn5 transposon mutant of P . carotovorum ( KD 200) was isolated that is highly increased in activity and transcript levels of extracellular cell wall‐degradative enzymes, pectate lyase, polygalacturonase, cellulase and protease. Accordingly, the mutant was more virulent as it macerated more host tissue than its parent. KD 200 mutant harbours a Tn5 insertion directly upstream of nss A Ecc71 , a putative sodium/sulphate symporter gene and member of a family of divalent anion sodium symporters ( DASS ). The findings reported here suggest that DASS proteins may be involved in soft rot pathogenesis.