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Effects of the biocontrol agent Bacillus amyloliquefaciens SN16‐1 on the rhizosphere bacterial community and growth of tomato
Author(s) -
Wan Tingting,
Zhao Huihui,
Wang Wei
Publication year - 2018
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12690
Subject(s) - rhizosphere , biology , bacillus amyloliquefaciens , fusarium wilt , fusarium oxysporum , terminal restriction fragment length polymorphism , rhizobacteria , pseudomonas fluorescens , fusarium , biological pest control , fusarium oxysporum f.sp. lycopersici , pseudomonas , pathogen , inoculation , botany , horticulture , microbiology and biotechnology , bacteria , restriction fragment length polymorphism , genotype , gene , biochemistry , genetics
Fusarium oxysporum is a common soil‐borne pathogen that causes serious economic losses in tomato crops worldwide. The purpose of this study was to evaluate the influence of the bio‐control agents Bacillus amyloliquefaciens SN 16‐1 and Pseudomonas fluorescens SN 15‐2 and the pathogen Fusarium oxysporum f.sp. lycopersici ( FOL ) inoculation on tomato rhizosphere bacterial communities and growth, as measured by terminal restriction fragment length polymorphism (T‐ RFLP ). Treatment with SN 16‐1 and SN 15‐2 had a transient influence on indigenous bacterial communities, with SN 16‐1 showing great potential for controlling FOL . The corresponding genera of terminal restriction fragments (T‐ RF s) that were significantly altered after 10 days were obtained using Ribosomal Database Project ( RDP ) database comparison. Genera that produce antibiotics and promote plant growth were activated by SN 16‐1 and FOL treatments, indicating that SN 16‐1 responds quickly to FOL invasion. Moreover, the bioremediation activity characteristic of certain genera and the levels of enzymes that degrade pathogen cell walls were decreased while bacterial nutrient cycling and plant growth promotion were enhanced with FOL treatment. In conclusion, we found that SN 16‐1 possesses the capacity to control tomato wilt, acts synergistically with soil microbes and does not have a persistent effect on the rhizosphere bacterial communities of tomato.