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A COI 1 gene in wheat contributes to the early defence response against wheat powdery mildew
Author(s) -
Liu Xiaoying,
Wang Junya,
Fan Baoli,
Shang Yuntao,
Sun Yifan,
Dang Chen,
Xie Chaojie,
Wang Zhenying,
Peng Yongkang
Publication year - 2018
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12667
Subject(s) - biology , powdery mildew , blumeria graminis , gene , genetics , appressorium , gene silencing , botany , plant disease resistance
The hormone jasmonate ( JA ) signalling pathway is involved in diverse developmental processes in plants. COI 1 is a key regulator in JA pathway. Although COI 1 homologous proteins have been identified in a variety of crops, their regulatory mechanisms in wheat defence responses remain unknown. Here, we cloned COI 1 from disease resistant wheat Brock, designated Ta COI 1 . Ta COI 1 cDNA was 1988‐bp long, with an ORF encoding 593 deduced amino acids. The phylogenetic tree showed that Ta COI 1 was clustered in the same clade of monocotyledons. The predicted protein possesses an F‐box domain, several LRR s and a conserved AMN 1 domain. Its transcription was induced in the compatible and incompatible wheat by Blumeria graminis f. sp. tritici ( Bgt ) fungus, with differences observed in response time. Expression pattern analysis in the susceptible wheat variety Jing411 revealed that Ta COI 1 was up‐regulated after Bgt inoculation at 12‐hour postinoculation (hpi). While, in Brock its transcription was rapidly induced and reached a first peak at 2 hpi. These results suggest that Ta COI 1 may be involved in the early response to powdery mildew. The expression of Ta COI 1 was decreased by virus‐induced gene silencing. The rate of successful penetration by Bgt was higher in all Ta COI 1 ‐silenced Brock, while the percentage of macrocephalic appressoria and resistance reactions was lower than control. These results suggest that silencing of the Ta COI 1 gene increased Brock susceptibility to infection. Together, these findings indicate that the Ta COI 1 gene could be involved in the early defence response against wheat powdery mildew and may play an important role in wheat– Bgt interactions.