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An in vitro investigation of Malaysian Phytophthora palmivora isolates and pathogenicity study on oil palm
Author(s) -
Mohamed Azni Intan Nur Ainni,
Sundram Shamala,
Ramachandran Vasagi,
Abu Seman Idris
Publication year - 2017
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12620
Subject(s) - phytophthora palmivora , biology , palm oil , inoculation , horticulture , oomycete , mycelium , palm , veterinary medicine , botany , pathogen , microbiology and biotechnology , phytophthora , agroforestry , medicine , physics , quantum mechanics
Bud rot disease affecting oil palm in South American countries is reported to be caused by Phytophthora palmivora . P. palmivora is a local pathogen affecting various crops in Malaysia, and this finding caused an alarm, which prompted an investigation of pathogenicity using Malaysian P. palmivora to assess the potentials of this Oomycete to infect oil palm in Malaysia. A total of 11 P. palmivora isolates were obtained from cocoa and durian for the study. Leaf bioassays via artificial inoculation using 50,000 zoospores/ml and mycelial agar disc showed severe necrotic lesions on the infection spot of oil palm (DxP) spear leaves. Mild infection was observed in oil palm spear leaves of OxG hybrids indicating lower susceptibility against P. palmivora infection. Phylogenetic analysis using internal transcribed spacer ( ITS ) data revealed that Malaysian isolates were genetically similar to Colombian isolates supported by significant bootstrap values. The leaf bioassay results revealed that Malaysian oil palm materials are susceptible towards local P. palmivora infection. The Colombian P. palmivora isolates causing bud rot incidence may have evolved over a long period of time, undergone sequential genetic shift to become more virulent towards Colombian oil palm planting materials.