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Identification and validation of reference genes for RT ‐ qPCR analysis in banana ( Musa spp.) under Fusarium wilt resistance induction conditions
Author(s) -
Zhang Lucheng,
Liu Lan,
Cheng Ping,
Shen Hanguo,
Rong Biao,
Liu Wen,
Yu Guohui
Publication year - 2017
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12614
Subject(s) - biology , fusarium wilt , reference genes , fusarium oxysporum , gene , cultivar , inoculation , plant disease resistance , gene expression , fusarium , microbiology and biotechnology , horticulture , genetics
Banana ( Musa spp.) is severely damaged by Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc). Biocontrol by inducing systemic resistance has been considered as one of the most important strategies to improve plant health. Very few studies have investigated appropriate reference gene selection for RT‐qPCR (quantitative real‐time polymerase chain reaction) analysis suitable for conditions of systemic activated resistance. In this study, we assessed over a time‐course the expression of seven candidate reference genes ( EF1 , TUB , ACT1 , ACT2 , L2 , RPS2 and RAN ) for Cavendish cultivar Brazilian ( Musa spp. AAA) and dwarf banana cultivar Guangfen No. 1 ( Musa spp. ABB) that were inoculated by Bacillus subtilis strain TR21 and Foc. We choose these plants because they are commonly planted in Southern China. Expression stability of the candidate genes was evaluated using various software packages (GeNorm, NormFinder and BestKeeper). L2 and TUB genes displayed maximum stability in Guangfen No. 1. In Brazilian, ACT1 and TUB were the most stable genes. To further validate the suitability of the reference genes identified in this study, the expression of pathogenesis‐related 1 ( PR1 ) gene under TR21 and Foc strains Foc004/Foc009 treatments was also studied. Identified reference genes in this work that are most suitable for normalizing gene expression data in banana under Fusarium wilt resistance induction conditions will contribute to the understanding of disease resistance mechanisms induced by biocontrol strains in banana.