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Mutations of the β‐tubulin gene fragments from carbendazim‐resistant isolates of Pestalotiopsis sp. causing strawberry leaf blight in Chiang Mai, Thailand
Author(s) -
Kummanid Jitparpat,
Akimitsu Kazuya,
Nalumpang Sarunya
Publication year - 2017
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12588
Subject(s) - biology , carbendazim , gene , blight , genetics , valine , botany , fungicide , amino acid
Screening of field isolates of Pestalotiopsis sp. from strawberry leaf blight in Thailand identified 56 carbendazim‐resistant isolates. Of 56 isolates, 39 highly resistant ( HR ) phenotypes grew well on PDA amended with carbendazim even at ≥500 mg/L. Isolates with carbendazim‐resistant phenotype had a conspicuous mutation at particular sites in the beta‐tubulin (β‐tubulin) gene sequence. A β‐tubulin encoding gene from this pathogen was cloned and sequenced. Analysis of the β‐tubulin gene in highly resistant ( HR ) isolates showed a substitution at codon 79 and 102 from serine ( AGT ) to lysine ( AAA ) and valine ( GTA ) to alanine ( GCA ), respectively. The detection of such point mutations in the β‐tubulin gene allows the rapid screening to detect carbendazim‐resistant isolates in the field.