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Antagonistic Yeasts for Biocontrol of the Banana Postharvest Anthracnose Pathogen Colletotrichum musae
Author(s) -
Zhimo V. Yeka,
Dilip Darsana,
Sten Jessica,
Ravat Vikas Kumar,
Bhutia Dawa Dolma,
Panja Birendranath,
Saha Jayanta
Publication year - 2017
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12533
Subject(s) - biology , postharvest , candida tropicalis , inoculation , biological pest control , spore , yeast , spore germination , pathogen , fungi imperfecti , microbiology and biotechnology , botany , horticulture , antifungal , biochemistry
The biocontrol potentials of Candida tropicalis YZ 1, C. tropicalis YZ 27 and Saccharomyces cerevisiae YZ 7 against the postharvest anthracnose pathogen Colletotrichum musae were investigated. Treatments with all the three biocontrol agents (1 × 10 8 CFU/ml) significantly reduced the natural anthracnose disease severity of harvested banana fruits stored at ambient condition. Germination and survival of C. musae spores were markedly inhibited by all the three yeast strains in in vitro tests. The niche overlap index ( NOI ) was used to determine the interaction between the antagonists and C. musae, and the results (high NOI values) suggest competitive exclusion of C. musae by the yeast strains. C. tropicalis YZ 27 inoculated on banana wounds exhibited rapid colonization and maintenance of its population on the inoculated site. The biocontrol efficacy was also observed as a function of concentration of the antagonist applied. The fruits treated with C. tropicalis YZ 27, 36 h before pathogen inoculation, showed the best results with 96.0% disease inhibition followed by those treated 24 h before with 84.0% inhibition. The above results point to competition for nutrients and space as the main mechanism of antagonistic action of C. tropicalis YZ 27 against C. musae .