Premium
Overexpression of Jatropha curcas Defensin ( JcDef ) Enhances Sheath Blight Disease Resistance in Tobacco
Author(s) -
Wang Qingwei,
Zhu Shuhua,
Liu Yao,
Li Rui,
Tan Shancai,
Wang Shenghua,
Tang Lin,
Chen Fang
Publication year - 2017
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12531
Subject(s) - biology , defensin , jatropha curcas , rhizoctonia solani , plant disease resistance , gene , transgene , botany , microbiology and biotechnology , genetics
Plant defensins are small, basic, cysteine‐rich peptides, belonging to the antimicrobial peptide superfamily, commonly found in the plant kingdom. In this study, we cloned and characterized a plant defensin gene from Jatropha curcas ( JcDef ). JcDef carried conserved receptor binding sites and a cysteine motif, and it was phylogenetically grouped together with defensin Ec‐ AMP ‐D2‐like in Elaeis guineensis . JcDef is localized to cytoplasm and highly expressed in young tissues with fast metabolism such as cotyledons and stem apexes. Transgenic expression of JcDef in tobacco showed enhanced resistance against sheath blight disease caused by R. solani , indicating the antibacterial function.