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Manganese Phosphite in Coffee Defence against Hemileia vastatrix , the Coffee Rust Fungus: Biochemical and Molecular Analyses
Author(s) -
Monteiro Ana Cristina Andrade,
Resende Mário Lúcio Vilela,
Valente Thaís Cainã Teixeira,
Ribeiro Junior Pedro Martins,
Pereira Vanessa Foresti,
Costa Josineide Rodrigues,
Silva Joyce Alves Goulart
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12525
Subject(s) - apx , biology , polyphenol oxidase , catalase , peroxidase , chitinase , inoculation , plant disease resistance , phenylalanine ammonia lyase , rust (programming language) , superoxide dismutase , horticulture , plantlet , botany , enzyme , biochemistry , gene , explant culture , computer science , programming language , in vitro
Phosphites (Phi) are inorganic salts of phosphorous acid that have been used as resistance elicitors to activate mechanisms underlying plant defence. This study assessed the effectiveness of manganese phosphite (MnPhi) for the management of rust ( Hemileia vastatrix ) on coffee seedlings. The effect of this compound on the induction of resistance was also measured by the expression of defence‐related genes such as POX (peroxidase) , CAT (catalase) , GLU ( β ‐1.3‐glucanase) and PAL (phenylalanine ammonia‐lyase) and by the activity of defence enzymes such as ascorbate peroxidase ( APX ), superoxide dismutase ( SOD ) and polyphenol oxidase ( PPO ). Foliar sprayings of MnPhi on coffee seedlings provided 63% control of rust severity. This compound induced defence responses in seedlings with increased transcription of genes POX , CAT , GLU and PAL in non‐inoculated plants and increased activity of APX , SOD and PPO enzymes in plants inoculated with H. vastatrix and in non‐inoculated plants. It is suggested that MnPhi can induce resistance in coffee seedlings.