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Genotypic and Pathotypic Diversity of Xanthomonas oryzae pv. oryzae Strains in Taiwan
Author(s) -
Deng WenLing,
Lin HengAn,
Shih YuCyuan,
Kuo ChienChih,
Tzeng JenYu,
Liu Liyu D.,
Huang ShuTzu,
Huang ChiMing,
Chung ChiaLin
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12495
Subject(s) - xanthomonas oryzae , biology , xanthomonas oryzae pv. oryzae , virulence , cultivar , genotype , genetic diversity , inoculation , veterinary medicine , xanthomonas , plant disease resistance , haplotype , genetics , horticulture , gene , population , medicine , demography , sociology
Rice bacterial leaf blight, caused by Xanthomonas oryzae pv. oryzae [(Ishiyama) Swings et al. 1990] ( Xoo ), is a major rice disease of the second crop season in Taiwan. A total of 88 Xoo strains collected from 10 major rice cultivating areas in Taiwan from 1986, 1997, 2000, 2004, and 2011 were characterized by repetitive‐element PCR ( REP ‐ PCR ) fingerprinting and virulence analyses. Among the five genetic clusters identified by the pJEL 1/ pJEL 2 ( IS 1112 ‐based) and REP 1R‐Dt/ REP 2‐D [repetitive extragenic palindromic ( REP )‐based] primer sets, clusters A, C and D contained Xoo strains from geographically distant regions, which suggests a high frequency of Xoo dispersal in Taiwan. The 88 Xoo strains were evaluated by inoculations on IRBB near‐isogenic lines and five Taiwan rice cultivars. A subset of 45 moderately or highly virulent strains were classified into 15 pathotypes by their compatible or incompatible reactions on IR 24 and 12 IRBB near‐isogenic lines, each containing a single resistance gene. Analysis of molecular haplotypes and pathotypes revealed a partial relationship. IRBB 5, IRBB 21 and IRBB 4 were incompatible with 96%, 96% and 73% of the strains, so xa5 , Xa21 and Xa4 can recognize most of the Xoo strains in Taiwan and elicit resistance. In contrast, IRBB 3 ( Xa3 ), IRBB 8 ( xa8 ), IRBB 10 ( Xa10 ), IRBB 11 ( Xa11 ), IRBB 13 ( xa13 ) and IRBB 14 ( Xa14 ) were susceptible to almost all of the 45 Xoo strains. Inoculation trials revealed significant differences in the susceptibility of five Taiwan cultivars to Xoo (from high to low susceptibility: Taichung Sen 10 > IR 24, Taichung Native 1 > Taichung 192, Taikeng 9, Tainan 11). This study provides useful information for resistance breeding and the development of disease management strategies against bacterial blight disease of rice.