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Expanded Strain‐Specific RT ‐ PCR Assay for Differential Detection of Currently Known Citrus Tristeza Virus Strains: a Useful Screening Tool
Author(s) -
Cook Glynnis,
Vuuren Stephanus P.,
Breytenbach Johannes H. J.,
Burger Johan T.,
Maree Hans J.
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12454
Subject(s) - strain (injury) , biology , citrus tristeza virus , genotype , population , virology , real time polymerase chain reaction , virus , genetics , gene , plant virus , medicine , anatomy , environmental health
Abstract Genotypic characterization of Citrus tristeza virus ( CTV ) strains has progressed significantly, but their phenotypic expression is poorly established as CTV naturally occurs as mixed‐strain populations. A screening system for the analysis of mixed‐strain populations is required for population studies and the correlation with symptom expression. In this study, a published CTV strain‐specific detection assay was expanded and improved to facilitate detection of currently known CTV strains. Supplementary RT ‐ PCR assays were developed for two variant groups of the RB strain and the HA 16‐5 strain, and assays for the T36 strain and generic CTV detection were improved. The value of the strain‐specific assays was shown by the ability to identify the strain components of two CTV cross‐protecting sources, GFMS 35 and LMS 6, used in the South African budwood certification scheme and to demonstrate the segregation of strains in budwood source trees.