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Differentiation and Classification of Phytoplasmas Associated with Almond and GF ‐677 Witches'‐Broom Diseases using rRNA and rp Genes
Author(s) -
Motamedi Ehsan,
Salehi Mohammad,
Sabbagh Seyed Kazem,
Salari Mohammad
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12448
Subject(s) - phytoplasma , broom , biology , restriction fragment length polymorphism , 16s ribosomal rna , nested polymerase chain reaction , phylogenetic tree , primer (cosmetics) , ribosomal rna , polymerase chain reaction , genetics , gene , ecology , chemistry , organic chemistry
Stone fruits are affected by several diseases associated with plant pathogenic phytoplasmas. Previous studies have been shown that phytoplasma agents of almond and GF ‐677 witches'‐broom (Alm WB and GWB , respectively) diseases belong to pigeon pea witches'‐broom (16Sr IX ) phytoplasma group. In this study, partial biological and molecular characterization was used to compare and classify phytoplasma agents of Khafr Alm WB ( KA lm WB ) and Estahban GWB ( EGWB ) diseases. Production of different symptoms in periwinkle indicated that agents of KA lm WB and EGWB are differentiable. Expected fragments were amplified from diseased almond and GF ‐677 trees in direct PCR using phytoplasma universal primer pairs P1/P7 and rpF1/rpR1 and nested PCR using P1/P7 followed by R16F2n/ R16R2 primer pair. 16S‐ rDNA Restriction fragment length polymorphism ( RFLP ) as well as phylogenetic analysis of rplV‐rpsC and 16S–23S rRNA spacer region sequences classified KA lm WB and EGWB phytoplasmas within 16Sr IX ‐C (rp IX ‐C) and 16Sr IX ‐B (rp IX ‐B) subgroups, respectively.