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Complete Genome Sequence of a Chinese Isolate of Melon Necrotic Spot Virus and its Phylogenetic Relationship
Author(s) -
Wu Huijie,
Wen Shaohua,
Hong Ni,
Veronica Truniger,
Gu Qinsheng
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12447
Subject(s) - biology , phylogenetic tree , genetics , start codon , stop codon , nucleic acid sequence , genome , virology , whole genome sequencing , gene , virus , sequence (biology) , genomic organization , nucleotide
The full‐length nucleotide sequence and genomic organization of a melon necrotic spot virus isolate from Haimen, China ( MNSV ‐ HM ), were determined. The MNSV ‐ HM genome consists of a positive sense single‐stranded RNA , 4267 nt in length, with at least five open reading frames ( ORF s) encoding p29, p89, p42, and two small 7‐ kD a proteins (p7A and p7B). p89 shares a common start codon with p29 and continues through the amber stop codon of p29 to produce an 89‐ kD a protein. The p7A ORF terminates in an amber codon whose read‐through could generate a 14‐ kD a protein. Phylogenetic analyses based on the p42 amino acid sequence and complete genomic sequence placed MNSV ‐ HM and Spanish isolates of the virus in a major cluster, indicating a close genetic relationship. In conclusion, we report the full‐length sequence of MNSV ‐ HM and its translation strategy. The obtained genomic organization and phylogenetic trees indicate that MNSV ‐ HM belongs to the MNSV genus Carmovirus . To the best of our knowledge, this is the first demonstration of the complete nucleotide sequence of an MNSV isolate from China.