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Molecular Characterization of Divergent Strawberry Mild Yellow Edge Virus Isolates from Eastern Canada
Author(s) -
Bhagwat Basdeo,
Dickison Virginia,
Su Li,
Bernardy Mike,
Wiersma Paul A.,
Nie Xianzhou,
Xiang Yu
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12444
Subject(s) - biology , phylogenetic tree , nova scotia , virus , strain (injury) , phylogenetics , genetics , recombination , genome , virology , gene , archaeology , anatomy , history
The complete genome sequences of four new isolates of strawberry mild yellow edge virus ( SMYEV ) were determined and analysed. The isolates, designated as AB 41‐01, AB 41‐02, NB 1165 and NS 26, were found from strawberry fields showing strawberry decline symptoms in eastern Canada. AB 41‐01 and AB 41‐02 were from a single‐plant sample originating from Prince Edward Island, while NB 1165 came from New Brunswick and NS 26 from Nova Scotia. Nucleotide sequence identities are 95.8% between AB 41‐01 and NB 1165, 99.6% between AB 41‐02 and NS 26, and 84% between AB 41‐01/ NB 1165 and AB 41‐02/ NS 26. The four isolates share nucleotide sequence identities of 83.5–89.9% to two previously identified SMYEV isolates, namely MY 18 and D7. Phylogenetic analysis indicates that the four Canadian isolates represented two new SMYEV strain types and the strain divergences were not likely from recombination events among all presently known SMYEV isolates.