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Multigene Sequence Analysis of Aster Yellows Phytoplasma Associated with Primrose Yellows
Author(s) -
Fránová Jana,
Přibylová Jaroslava,
Koloniuk Igor,
Podrábská Kateřina,
Špak Josef
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12442
Subject(s) - biology , phytoplasma , aster yellows , ribosomal rna , ribosomal protein , genetics , restriction fragment length polymorphism , microbiology and biotechnology , 16s ribosomal rna , gene , ribosomal dna , polymerase chain reaction , phylogenetics , ribosome , rna
Primula acaulis (L.) Hill. plants showing stunting, leaf‐yellowing and virescence were first discovered in the Czech Republic. Polymerase chain reactions with subsequent restriction fragment length polymorphism analyses and sequencing enabled classification of the detected phytoplasmas into the aster yellows group, ribosomal subgroup 16SrI‐B, tuf I‐B, rp I‐B, gro ELIB ‐ III and SecY‐ IB subgroups. Phylogeny of the 16S rRNA gene sequences as well as sequence analysis of several chromosomal regions, such as the 16S‐23S ribosomal operon, ribosomal proteins, spc ribosomal protein operon, genes for elongation factor EF ‐Tu, molecular chaperonin large subunit Gro EL , immunodominant membrane protein, ribosome recycling factor, urydilate kinase, ATP ‐ and Zn 2+ ‐dependent proteases not only confirmed its affiliation with the ‘ Candidatus Phytoplasma asteris’ species but also enabled its detailed molecular characterization. The less researched regions of phytoplasma genome ( amp , adk , hflB , pyrH ‐ frr genes) could be valuable as additional markers for phytoplasma through differentiation especially within the 16SrI‐B ribosomal subgroup.