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New Host Record of Myrothecium roridum Causing Leaf Spot on  Abutilon megapotamicum from China
Author(s) -
Ben HaiYan,
Gao Wei,
Qu HongYun,
Chai ALi,
Shi YanXia,
Xie XueWen,
Li BaoJu
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12439
Subject(s) - biology , leaf spot , abutilon , internal transcribed spacer , botany , host (biology) , pathogenicity , spots , gene , ribosomal rna , microbiology and biotechnology , genetics , weed
During September 2010, Abutilon megapotamicum plants with dark‐brown concentric spots on leaves were observed in a commercial glasshouse located in Beijing, China. This study was carried out to identify the causal agent of this disease based on Koch's postulates and morphological characteristics. Pathogenicity tests in the glasshouse showed that Myrothecium roridum Tode ex Fr. caused the leaf spot on A. megapotamicum plants, which were the same as those observed in naturally infected plants in the field. Moreover, to confirm the pathogen to species, the rDNA internal transcribed spacer ( ITS ) of isolate was PCR ‐amplified using ITS 1 and ITS 4 primer pairs and sequenced. DNA analysis revealed a 100% species identity index for M. roridum . To the best of our knowledge, this is the first report of M. roridum on A. megapotamicum in China.

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