Premium
Rapid Discrimination between Groups I and II of Acidovorax citrulli Using a Primer Pair Specific to a pil L Gene
Author(s) -
Zhong Jie,
Lin ZhenYa,
Ma YaMin,
Gao BiDa,
Liu HuiQin,
Zhao TingChang,
Schaad Norman W.
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12435
Subject(s) - biology , primer (cosmetics) , multilocus sequence typing , polymerase chain reaction , pulsed field gel electrophoresis , microbiology and biotechnology , gel electrophoresis , gene , strain (injury) , genetics , genotype , chemistry , organic chemistry , anatomy
Acidovorax citrulli can be divided into two genetic groups: group I and group II based primarily on pulsed‐field gel electrophoresis ( PFGE ) and multilocus sequence classification ( MLST ). To distinguish more rapidly between strains of the two groups, a pair of specific primer for specific polymerase chain reaction ( PCR ) that can identify group II strains was designed based on the pil L gene of a group II strain, AAC 00‐1. PCR results showed that a 332‐bp band was generated for 51 of 52 group II strains whereas only three of 93 group I strains were positive, largely consisting with previous studies of A. citrulli classification. Results of PCR showed the primers were able to detect group II strains of A. citrulli and distinguish between strains of groups I and II rapidly and accurately.