z-logo
Premium
Complete Nucleotide Sequence of Ageratum enation virus and an Alphasatellite Infecting a New Host Glycine max in India
Author(s) -
Sharma Shivani,
Singh Lakhmir,
Roshan Poonam,
Kulshreshtha Aditya,
Singh Nageswar,
Hallan Vipin
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12434
Subject(s) - ageratum conyzoides , biology , begomovirus , nucleic acid sequence , geminiviridae , virology , weed , polymerase chain reaction , rolling circle replication , genome , nucleotide , dna , virus , plant virus , genetics , gene , botany , dna polymerase
During 2011, leaf crumpling, yellowing and stunting were observed on soya bean ( Glycine max ) in Himachal Pradesh, India. PCR‐based detection confirmed the presence of a begomovirus. The viral genome was amplified by rolling circle amplification, cloned and sequenced. The complete nucleotide sequence of DNA‐A showed highest nucleotide identity to an isolate of Ageratum enation virus infecting a weed Ageratum conyzoides . In addition, a DNA molecule was found which shared 95% nucleotide identity with an alphasatellite infecting ageratum. Neither beta satellite nor DNA‐B was detected in the infected samples.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here