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Complete Nucleotide Sequence of Ageratum enation virus and an Alphasatellite Infecting a New Host Glycine max in India
Author(s) -
Sharma Shivani,
Singh Lakhmir,
Roshan Poonam,
Kulshreshtha Aditya,
Singh Nageswar,
Hallan Vipin
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12434
Subject(s) - ageratum conyzoides , biology , begomovirus , nucleic acid sequence , geminiviridae , virology , weed , polymerase chain reaction , rolling circle replication , genome , nucleotide , dna , virus , plant virus , genetics , gene , botany , dna polymerase
During 2011, leaf crumpling, yellowing and stunting were observed on soya bean ( Glycine max ) in Himachal Pradesh, India. PCR‐based detection confirmed the presence of a begomovirus. The viral genome was amplified by rolling circle amplification, cloned and sequenced. The complete nucleotide sequence of DNA‐A showed highest nucleotide identity to an isolate of Ageratum enation virus infecting a weed Ageratum conyzoides . In addition, a DNA molecule was found which shared 95% nucleotide identity with an alphasatellite infecting ageratum. Neither beta satellite nor DNA‐B was detected in the infected samples.