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Partial Sequence Analysis of Geographically Close Grapevine virus A Isolates Reveals their High Regional Variability and an Intra‐Isolate Heterogeneity
Author(s) -
Predajňa Lukáš,
Glasa Miroslav
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12432
Subject(s) - biology , capsid , genetic diversity , phylogenetic tree , genetic variability , population , sequence analysis , sequence (biology) , genetics , genetic variation , virus , gene , genotype , demography , sociology
To evaluate the genetic diversity of Grapevine virus A ( GVA ), the genomic region encompassing the partial capsid protein gene and ORF 5 was analysed from 10 GVA isolates. Phylogenetic study showed a broad variability of the GVA isolates recovered from a limited geographical area (Slovakia and Czech Republic) and further confirmed the absence of geographical structuration within GVA . Moreover, assessment of structure and intra‐isolate variability revealed that grapevine samples infected with SK 13 and SK 29 isolates have harboured a population of different sequence variants.