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Identification of the New Pathogen ( Stemphylium lycopersici ) Causing Leaf Spot on Pepino ( Solanum muricatum )
Author(s) -
Nasehi Abbas,
Kadir Jugah,
NasrEsfahani Mehdi,
AbedAshtiani Farnaz,
Golkhandan Elham,
Ashkani Sadegh
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12431
Subject(s) - biology , leaf spot , solanum , pathogen , solanaceae , botany , internal transcribed spacer , pathogenicity , spots , horticulture , microbiology and biotechnology , ribosomal rna , gene , genetics
Pepino ( Solanum muricatum var . pepino ) plants were found affected by an extensive leaf spot caused by plant pathogenic fungi during a survey in the Cameron highlands, Pahang state, Malaysia. Symptomatic leaf samples were collected from infected pepino plants and cultivated on PDA medium, and the pathogen was isolated and purified; then, consequently, all isolates were identified as Stemphylium lycopersici on the basis of their cultural and morphological characteristics and combined sequences of the internal transcribed spacer ( ITS ) and glyceraldehyde‐3‐phosphate dehydrogenase ( gpd ) regions. A pathogenicity assay on detached leaves further confirmed that S. lycopersici causes leaf spot disease. To the best of our knowledge, this is the first report of S. lycopersici causing leaf spot on pepino in Malaysia and worldwide.