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Biological and Molecular Characterization of Alfalfa Mosaic Virus Infecting Trumpet Creeper ( Campsis radicans ) in Iran
Author(s) -
Pourrahim Reza,
Farzadfar Shirin
Publication year - 2016
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12416
Subject(s) - biology , alfalfa mosaic virus , genbank , virology , virus , vigna , plant virus , phaseolus , cucumber mosaic virus , nicotiana tabacum , botany , gene , genetics , coat protein , rna
Samples of trumpet creeper ( Campsis radicans ) leaves showing mottling and mosaic were collected from plants growing in a private garden in Tehran province, Iran, in 2012. Symptomatic leaf samples were tested for Alfalfa mosaic virus ( AMV ), Cucumber mosaic virus ( CMV ) and Peanut stunt virus ( PSV ) infection in enzyme‐linked immunosorbent assay ( ELISA ), using specific antibodies. None of the samples were positive for CMV and PSV ; however, all reacted positively with that of AMV antiserum. In biological assay, systemic infection was found on Datura stramonium , Nicotiana tabacum cvs., White Burley, and Xanthi, 21 days postinoculation ( DPI ), while necrotic local lesions were obtained following inoculation of Phaseolus vulgaris and Vigna unguiculata within three to four DPI . Using a pair of primers specific for AMV , a DNA fragment of 880 bp was RT ‐ PCR ‐amplified. Analysis of the sequences revealed the presence of 657 nucleotides of AMV complete coat protein ( CP ) gene (translating 218 amino acid residues). Phylogenetic analysis using neighbour‐joining ( NJ ) method clustered AMV isolates into two main types and the IRN ‐Tru (GenBank Accession No. JX865593 ) isolate fell into type I. Pairwise nucleotide distances also confirmed two main types with the highest and lowest similarities for type I and II, respectively. The association of AMV with mosaic disease of C. radicans represents the first record from the world.