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Differential Colonization Patterns of Bananas ( Musa spp.) by Physiological Race 1 and Race 4 Isolates of Fusarium oxysporum f.sp. cubense
Author(s) -
Guo Lijia,
Yang Laying,
Liang Changcong,
Wang Guofen,
Dai Qingdon,
Huang Junsheng
Publication year - 2015
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12378
Subject(s) - fusarium oxysporum f.sp. cubense , biology , fusarium oxysporum , colonization , fusarium wilt , virulence , inoculation , spore , xylem , musaceae , race (biology) , microbiology and biotechnology , botany , gene , horticulture , genetics
Fusarium oxysporum f.sp. cubense (Foc) is the causative agent of Fusarium wilt of bananas ( Musa spp.). To clarify the colonization patterns of Foc in bananas, two green fluorescent protein‐tagged isolates, NT 320 (race 1) and B2‐gfp (race 4), were used to follow infection of the banana varieties Pisang Awak and Brazil. Penetration and colonization of both isolates in roots of these two banana varieties were observed within 6 days, but sporulation in xylem vessels was not observed until day 30 postinoculation. Interestingly, B2‐gfp penetrated into xylem vessels of Pisang Awak banana roots more quickly than NT 320, implying that the race 4 isolate is more virulent than the race 1 isolate. This result was further confirmed by comparing the disease severity of plants inoculated with NT 320 with that of plants inoculated with B2‐gfp. Quantitative real‐time PCR revealed that some pathogenicity‐associated genes, including Fga1 , Fhk1 , Fow2 and Ste12 , were upregulated by B2‐gfp during exposure to Brazil bananas, while they were either downregulated by NT 320 or not significantly changed. These data might partly explain why the race 4 isolate was more virulent than the race 1 isolate.