Premium
Pathogenic and Genetic Variation among the Isolates of Rhizoctonia solani ( AG 1‐ IA ), the Rice Sheath Blight Pathogen
Author(s) -
Yugander Arra,
Ladhalakshmi Duraisamy,
Prakasham Vellaichamy,
Mangrauthia Satendra K.,
Prasad Madamsetty S.,
Krishnaveni Donempudi,
Madhav Maganti Sheshu,
Sundaram Raman M.,
Laha Gouri Sankar
Publication year - 2015
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12343
Subject(s) - biology , rhizoctonia solani , pathogen , virulence , rhizoctonia , sheath blight , veterinary medicine , poaceae , blight , horticulture , genetic variation , botany , microbiology and biotechnology , gene , genetics , medicine
Sheath blight disease of rice caused by Rhizoctonia solani is one of the most dreaded plant diseases faced by the rice farmers all over the world. None of the commercially cultivated rice varieties have sufficient level of field resistance, and the disease is presently being managed by chemical pesticides. In this study, 40 isolates of rice sheath blight pathogen, collected from diverse rice ecosystems from 12 different states of India, were characterized for their morphological, pathological and genetic variation. The isolates showed wide morphological variation in terms of size of sclerotia and abundance of sclerotia production. The virulence of each pathogen isolate was studied on four rice varieties, that is TN 1, IR 64, Tetep and Swarnadhan in glasshouse, and observations were taken by measuring the relative lesion height. The relative lesion heights produced by these isolates on four different rice varieties varied widely. Genetic variation of the isolates was analysed using ISSR markers. The primers based on AG , GA , AC and CA repeats were informative and revealed polymorphism among the isolates. The polymorphism information content ( PIC ) of the primers ranged from 0.80 to 0.96, while the resolving power ( Rp ) ranged from 3.7 to 15.35. Largely, grouping of the isolates happened based on their geographical origin. One isolate from Titabar, Assam, and another from Adialabad, Telangana, were quite distinct from rest of the isolates.