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Analysis of Pathogenic Diversity of the Rice Bacterial Blight Pathogen ( Xanthomonas oryzae pv. oryzae ) in the Andaman Islands and Identification of Effective Resistance Genes
Author(s) -
Gautam Raj K.,
Singh Pankaj K.,
Sakthivel Krishnan,
Srikumar Muthulingam,
Kumar Naresh,
Kumar Krishna,
Singh Awnindra K.,
Roy Sibnarayan Dam
Publication year - 2015
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12338
Subject(s) - biology , xanthomonas oryzae , xanthomonas oryzae pv. oryzae , rapd , virulence , genetic diversity , blight , gene , pathogen , genetics , context (archaeology) , plant disease resistance , bacterial blight , microbiology and biotechnology , veterinary medicine , botany , population , medicine , paleontology , demography , sociology
Bacterial blight ( BB ) caused by X anthomonas oryzae pv. oryzae ( X oo ) is a major disease of rice in the tropics for which genetic resistance in the host plants is the only effective solution. This study aimed at identification of resistance gene combinations effective against X oo isolates and fingerprinting of the X oo isolates of A ndaman I slands ( I ndia). Here, we report the reaction of 21 rice BB differentials possessing X a1 to X a21 genes individually and in different combinations to various isolates of pathogen collected from A ndaman I slands. Pathological screening results of 14 isolates revealed that among individual genes tested across 2 years, X a4, X a7 and X a21 conferred resistance reaction across all isolates, whereas among combinations, IRBB 50 ( Xa4 + xa5 ), IRBB 52 ( Xa4 + Xa21 ) and IRBB 60 ( Xa4 + xa5 + xa13 + Xa21 ) conveyed effective resistance against tested isolates. The nature of genetic diversity among four isolates selected on the basis of geographical isolation in the islands was studied through DNA finger printing. The RAPD primers S 111, S 119, S 1117, S 1109, S 1103, S 109 and S 105 were found to be better indicators of molecular diversity among isolates than JEL primers. The diversity analysis grouped 14 isolates into three major clusters based on disease reaction wherein isolate no. 8 was found the most divergent as well as highly virulent. The remaining isolates were classified into two distinct groups. The importance of the study in the context of transfer of resistance gene(s) in the local cultivars specifically for tropical island conditions is presented and discussed.