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Molecular Detection and Characterization of Chinese Yam Mild Mosaic Virus Isolates
Author(s) -
Wang Mingqiang,
Li Fan,
Zhou Guohui,
Lan Pingxiu,
Xu Donglin,
Li Ruhui
Publication year - 2015
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12337
Subject(s) - biology , phylogenetic tree , genetics , mosaic virus , coat protein , mosaic , nucleic acid sequence , virus , virology , plant virus , gene , rna , history , archaeology
An improved RT ‐ PCR was developed and validated for the detection of Yam mild mosaic virus ( YMMV ). Sequences of the coat protein core region of 19 Chinese isolates were obtained, and analysis indicated the presence of different genetic variants. Phylogenetic analyses showed that the Chinese isolates were divided into two distinct clusters. Complete genomic sequences of two distinct Chinese variants were determined to be 9527 and 9529 nucleotides long, excluding the 3′ poly (A) tail. Their genomic structure and organization were virtually identical to that of a Brazilian isolate. The two variants shared identity of 87.3% to one another and 83.9–84.6% to the Brazilian variant at the genomic sequence level. Phylogenetic analyses supported that they represented two distinct YMMV lineages.