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Bougainvillea Shoot Proliferation, a New Disease Induced by Distinct Phytoplasmas
Author(s) -
Silva Eliane Gonçalves,
Flôres Daniela,
Bedendo Ivan Paulo
Publication year - 2015
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12325
Subject(s) - phytoplasma , biology , shoot , phylogenetic tree , botany , chlorosis , bract , restriction fragment length polymorphism , horticulture , polymerase chain reaction , genetics , gene , inflorescence
Bougainvillea‐potted plants exhibiting typical phytoplasma‐induced symptoms, characterized by foliar chlorosis, shoot proliferation, leaf and bract deformations, and decline were observed in commercial nurseries, located in the state of S ão P aulo, B razil. In this study, PCR assays using group‐specific primers revealed that phytoplasmas affiliated with the groups 16SrI and 16Sr III were associated with symptomatic plants. Molecular analysis based on conventional and virtual RFLP patterns and similarity coefficient calculations identified these phytoplasmas as belonging to subgroups 16SrI‐B and 16Sr III ‐B. Phylogenetic analysis confirmed that these phytoplasmas were closely related to representatives of both subgroups. Transmission assays using dodder supported the initial evidence that the symptoms were associated with phytoplasmas.