Occurrence of a New Orange Variant of C urtobacterium flaccumfaciens pv . flaccumfaciens , Causing Common Bean Wilt in I ran

A new orange variant of C urtobacterium flaccumfaciens pv . flaccumfaciens was isolated from seeds of common bean cv. D aneshkadeh and D ehghan stored in the seed banks in K homein B ean R esearch S tation, and field plants (cv. L ocal K homein) in A rak, I ran. The pathogenicity of the isolates was confirmed on 5‐ to 7‐day‐old seedlings of cv. D aneshkadeh. Marginal necrosis and interveinal chlorosis on first trifoliate leaves were observed 10–15 days after inoculation. Amplification of 306 bp fragment of orange‐pigmented strains using C ff FOR 2‐ and C ff REV 4‐specific prime pair characterized them as C . flaccumfaciens pv. flaccumfaciens . Although the yellow‐pigmented variant of the causal agent was previously reported on cowpea, this is the first report of orange variant of C . flaccumfaciens pv. flaccumfaciens causing bacterial wilt on common bean in I ran.