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Identification and Analysis of Powdery Mildew‐Responsive mi RNA s in Wheat
Author(s) -
Wu Fangli,
Guo Qingli,
Zhang Wei,
Jin Weibo
Publication year - 2015
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12315
Subject(s) - powdery mildew , blumeria graminis , biology , rna , microrna , common wheat , transcriptome , gene expression , genetics , gene , mildew , small rna , botany , plant disease resistance , chromosome
Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt) , is one of the most consistently damaging diseases of common wheat worldwide and greatly affects crop productivity. Recently, several plant micro RNA s (mi RNA s) have been reported as gene expression regulators related to various adverse environments. However, up to now, less is known on the roles of mi RNA s in powdery mildew infection response of wheat. In this study, mi RNA expression patterns were investigated for identifying Bgt ‐responsive mi RNA s in wheat leaves using a plant mi RNA microarray platform. A total of 79 mi RNA s from 24 families were detected in wheat leaves. Among those, seven mi RNA s were further validated to be involved in wheat powdery mildew response and two of them have never been reported. In addition, their target expression profiles showed a negative correlation with that of the seven mi RNA s in mock‐ and Bgt ‐infected samples furtherly proved, which in turn as the robust evidence, that those seven powdery mildew‐responsive mi RNA s are highly reliable. These findings could extend the current view about mi RNA s as ubiquitous regulators under stress conditions.