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Diagrammatic Scale for Assessment of Bacterial Blight in Coffee Leaves
Author(s) -
Belan Leônidas L.,
Pozza Edson A.,
Freitas Marcelo L. de O.,
Souza Ricardo M.,
Jesus Junior Waldir C.,
Oliveira Júlia M.
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12272
Subject(s) - repeatability , reproducibility , biology , diagrammatic reasoning , scale (ratio) , blight , visual analogue scale , veterinary medicine , statistics , mathematics , botany , medicine , computer science , surgery , cartography , programming language , geography
Due to the lack of a standardized visual method for assessing bacterial blight ( P seudomonas syringae pv. garcae ) in coffee leaves, a diagrammatic scale was developed and validated to quantify the disease. Leaves were collected in crops and nursery with different intensity of symptoms, and the true severity was determined electronically. Based on the frequency distribution of severity values and according to the W eber– F echner's law of visual stimulus, the minimum and maximum limits and the intermediate levels in the scale were determined. Validation was performed by ten evaluators who estimated the severity of 50 leaves with different intensity of symptoms. One evaluation was performed without diagrammatic scale and two evaluations with the scale at 7‐day intervals. The accuracy, precision, repeatability and reproducibility of the estimates were evaluated. The scale had nine levels: 0 (0%), 1 (0.1–0.99%), 2 (1–2%), 3 (2.01–4%), 4 (4.01–8%), 5 (8.01–16%), 6 (16.01–25%), 7 (25.01–45%) and 8 (≥45.1%). Using the scale, the evaluators were able to improve accuracy, precision, reproducibility and repeatability of estimates, compared to evaluators without scale. The scale was appropriate to visual estimation of severity of bacterial blight in coffee leaves.