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Purification of p47f, a Necrosis‐Inducing Protein Fraction from the Secretome of Phytophthora capsici
Author(s) -
FloresGiubi María Eugenia,
DíazBrito Mayra Alejandra,
BritoArgáez Ligia,
GarcíaSosa Karlina,
EscalanteErosa Fabiola,
PeñaRodríguez Luis Manuel,
IslasFlores Ignacio
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12271
Subject(s) - phytophthora capsici , biology , wilting , elicitor , oomycete , microbiology and biotechnology , protease , cycloheximide , necrosis , botany , pathogen , biochemistry , protein biosynthesis , phytophthora , enzyme , genetics
The oomycete P hytophthora capsici causes wilting disease in chilli pepper and another solanaceous plants, with important economic consequences. Although much investigation has been conducted about this pathogen, little is still known about which of its proteins are involved in the infection process. In this study, the bioassay‐guided fractionation of the secretome of P . capsici resulted in the purification of a phytotoxic protein fraction designated as p47f, capable of inducing wilting and necrosis on leaves of C apsicum chinense J acq, and having a 47 kD a polypeptide with proteolytic activity as the major component. The isolated p47f fraction induced DNA degradation and decreased cell survival of C . chinense cell suspension culture. Sequencing of p47f indicated the presence of 15 proteins, which could be grouped into seven classes including a protease group, cell wall remodelling proteins and the transglutaminase elicitor M 81 D , among others. This is the first report of P . capsici secreting proteins that modulate cell responses mediated by ROS in the host.