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Evidence of Cotton leaf curl Burewala virus Variant and its Associate Betasatellite Causing Yellow Mosaic of Eggplant ( Solanum melongena ) in Pakistan
Author(s) -
Ullah Rahim,
Akhtar Khalid P.,
Hassan Ishtiaq,
Saeed Muhammad,
Sarwar Nighat,
Mansoor Shahid
Publication year - 2015
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12268
Subject(s) - genbank , biology , melongena , begomovirus , solanum , leaf curl , accession number (library science) , plant virus , virology , virus , tomato yellow leaf curl virus , nucleic acid sequence , botany , horticulture , veterinary medicine , gene , genetics , medicine
Eggplant ( S olanum melongena L .) plants with severe leaf mosaic and mottling were found in a kitchen garden near cotton fields in P akistan. Rolling Circle Amplification products from six of the naturally infected eggplant plants, subjected to PCR , successfully amplified expected products of 2.8 and 1.4 kb using begomovirus and betasatellite‐specific primers, respectively. Based on 99% nucleotide sequence identity, the virus was identified as a variant of C otton leaf curl Burewala virus ( CLC u B u V ) (GenBank Accession No. HG428709). Likewise, the sequenced betasatellite with a maximum of 97% nucleotide sequence identity was recognized as a new variant of C otton leaf curl M ultan betasatellite ( CLC uMu B M ul ) (GenBank Accession No. HG428708). The symptomatic induction of C otton leaf curl disease in CLC uBuV susceptible cotton genotype CIM ‐496 by back‐indexing further confirmed the presence of CLC uBuV in eggplant. This is the first report of CLC uBuV and its associate betasatellite in naturally infected plants of eggplant.