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Molecular Characterization of Extrachromosomal DNA Accompanying Primula Red Phytoplasma
Author(s) -
Sarkisova Tatiana,
Lenz Ondřej,
Petrzik Karel
Publication year - 2015
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12265
Subject(s) - extrachromosomal dna , biology , phytoplasma , plasmid , genetics , primula , gene , open reading frame , genome , nucleic acid sequence , homology (biology) , botany , peptide sequence , restriction fragment length polymorphism , polymerase chain reaction
The complete nucleotide sequence of an extrachromosomal element found in primula red isolate of ‘ Candidatus Phytoplasma asteris’ (16SrI‐ B subgroup) was determined. The plasmid, named pPrR , is 4378 bp in length and has 75% A + T content that is similar to that of the phytoplasma genome. It encodes six putative open reading frames ( ORF ) longer than 100 amino acids and two smaller ones. The structural organization of the rep gene is similar to that found in plasmids which replicate via rolling circle mechanism. Furthermore, it has homology to both the plasmid pLS 1 family and helicase domains of replication‐associated proteins (Rap) of eukaryotic viruses and geminiviruses. The ORF arrangement and genes sequences are most similar to the pPARG 1 plasmid from ‘ Rehmannia glutinosa ’ phytoplasma.