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First Report of Phytoplasma (16SrI) Associated with Yellow Decline Disease of Royal Palms [ Roystonea regia (Kunth) O. F. Cook] in Malaysia
Author(s) -
Naderali Neda,
Vadamalai Ganesan,
Nejat Naghmeh,
Ling Kong L.
Publication year - 2015
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12262
Subject(s) - phytoplasma , biology , aster yellows , phyllody , restriction fragment length polymorphism , botany , 16s ribosomal rna , nested polymerase chain reaction , primer (cosmetics) , phylogenetic tree , frond , polymerase chain reaction , gene , genetics , chemistry , organic chemistry
Royal Palms ( Roystonea regia ) with symptoms such as severe chlorosis, stunting, collapse of older fronds and general decline were observed in the state of Selangor, Malaysia. Using polymerase chain reaction (PCR) amplification with phytoplasma universal primer pair P1/P7 followed by R16F2N/R16R2 and fU 5/ rU 3 as nested PCR primer pairs, all symptomatic plants tested positively for phytoplasma. Results of phylogenetic and virtual RFLP analysis of the 16S rRNA gene sequences revealed that the phytoplasma associated with Royal Palm yellow decline (RYD) was an isolate of ‘ Candidatus Phytoplasma asteris’ belonging to a new 16SrI‐subgroup. These results show that Roystonea regia is a new host for the aster yellows phytoplasma (16SrI). This is the first report on the presence of 16SrI phytoplasma on Royal Palm trees in Malaysia.