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Development of a Loop‐Mediated Isothermal Amplification Assay to Detect F usarium oxysporum
Author(s) -
Lu Chenchen,
Dai Tingting,
Zhang HaiFeng,
Wang YuanChao,
Zheng XiaoBo
Publication year - 2015
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12259
Subject(s) - loop mediated isothermal amplification , biology , microbiology and biotechnology , detection limit , chromatography , dna , biochemistry , chemistry
We report the development of a loop‐mediated isothermal amplification ( LAMP ) assay targeting the CYP 51C element for visual detection of F . oxysporum which caused F usarium wilt in soybean. The CYP 51C ‐ LAMP assay efficiently amplified the target gene in 60 min at 62°C. And specificity was evaluated against F . oxysporum , F usarium spp. and other fungal species. The detection limit of the CYP 51 C ‐specific LAMP assay for F . oxysporum was four conidia per gram soil. The assay also detected F . oxysporum from inoculated soybean tissues and residues. These results suggest that this CYP 51C ‐ LAMP assay can be used to detect residues on plants in the field.