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Detection and Molecular Identification of Aster Yellows Phytoplasma in Date Palm in E gypt
Author(s) -
AlKhazindar Maha
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12241
Subject(s) - phytoplasma , biology , phylogenetic tree , aster yellows , palm , accession number (library science) , nested polymerase chain reaction , polymerase chain reaction , botany , gene , horticulture , genetics , restriction fragment length polymorphism , genbank , physics , quantum mechanics
Phytoplasma‐like symptoms were detected in date palm trees ( P hoenix dactylifera L .) in A l‐ G iza G overnorate in E gypt. S ymptoms varied from leaf chlorotic streaks, stunting and marked reduction in fruit and stalk sizes. Direct and nested PCR of symptomatic samples using P 1/ P 7 and R 16 F 2n/ R 16 R 2n primers, respectively, of the 16 S r RNA gene, resulted in a DNA amplification product of c . 1.3 kbp. Symptomless samples collected from the same location and the healthy control produced no product upon amplification. Products were cloned into TOPO TA vector for sequencing. Data generated were deposited in the G en B ank (Accession KF826615 ). A BLAST search showed that the sequence of the 16 S r RNA gene shared ‘ C andidatus P hytoplasma asteris ’ (16 S r I group) with other isolates. Phylogenetic analysis revealed that the isolate clustered with the date palm phytoplasma causing A l‐ W ijam disease in S audi A rabia.