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Occurrence of R alstonia solanacearum Race 2 Biovar 1 Associated with Moko Disease of Banana ( M usa paradisiaca cv. N ipah) in M alaysia
Author(s) -
Zulperi Dzarifah,
Sijam Kamaruzaman,
Mior Ahmad Zainal A,
Awang Yahya,
Sulaiman Rashid Tavga
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12233
Subject(s) - biovar , biology , amplicon , phylogenetic tree , strain (injury) , microbiology and biotechnology , polymerase chain reaction , bacteria , gene , genetics , anatomy
During J une 2011 to M arch 2012, M oko disease symptoms were observed in banana cv. N ipah in two M alaysian states. The primer pairs ISR so19 F / ISR so19 R were used for defined identification of R alstonia solanacearum race 2 strain. PCR amplification of all isolates produced a 1900 amplicon and exhibited 93% phylogenetic similarity with reference strain ( AF 450275). Based on symptoms, biochemical tests, pathogenicity assay, molecular and phylogenetic studies, we concluded that the isolated bacterium was R . solanacearum race 2 biovar 1.