Occurrence of   R alstonia solanacearum  Race 2 Biovar 1 Associated with Moko Disease of Banana (  M usa paradisiaca  cv.  N ipah) in  M alaysia | Zendy

Zulperi Dzarifah | Zendy; Sijam Kamaruzaman | Zendy; Mior Ahmad Zainal A | Zendy; Awang Yahya | Zendy; Sulaiman Rashid Tavga | Zendy

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Occurrence of R alstonia solanacearum Race 2 Biovar 1 Associated with Moko Disease of Banana ( M usa paradisiaca cv. N ipah) in M alaysia

Author(s) -

Zulperi Dzarifah,

Sijam Kamaruzaman,

Mior Ahmad Zainal A,

Awang Yahya,

Sulaiman Rashid Tavga

Publication year - 2014

Publication title -

journal of phytopathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.53

H-Index - 60

eISSN - 1439-0434

pISSN - 0931-1785

DOI - 10.1111/jph.12233

Subject(s) - biovar , biology , amplicon , phylogenetic tree , strain (injury) , microbiology and biotechnology , polymerase chain reaction , bacteria , gene , genetics , anatomy

During J une 2011 to M arch 2012, M oko disease symptoms were observed in banana cv. N ipah in two M alaysian states. The primer pairs ISR so19 F / ISR so19 R were used for defined identification of R alstonia solanacearum race 2 strain. PCR amplification of all isolates produced a 1900 amplicon and exhibited 93% phylogenetic similarity with reference strain ( AF 450275). Based on symptoms, biochemical tests, pathogenicity assay, molecular and phylogenetic studies, we concluded that the isolated bacterium was R . solanacearum race 2 biovar 1.

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