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The Infection Process of P estalotiopsis Longisetula Leaf Spot on Strawberry Leaves
Author(s) -
Rodrigues Fabrício A.,
Silva Ivanete Tonole,
Antunes Cruz Maria Fernanda,
CarréMissio Vivian
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12226
Subject(s) - conidium , pycnidium , biology , hypha , inoculation , fungus , germination , botany , germ tube , penetration (warfare) , parenchyma , horticulture , operations research , engineering
Abstract Pestalotia leaf spot, caused by the fungus P estalotiopsis longisetula Guba, has become the major disease affecting strawberry production in B razil. Strawberry seedlings with 4–5 leaves were inoculated with a conidial suspension of P . longisetula (2 × 10 5 conidia/ml), and leaf samples were collected at 48, 72, 96 and 144 h after inoculation (hai) for observation in the scanning electron microscope. Conidia germinated within 48 hai. At 72 hai, conidia had formed very long germ tubes over the epidermal cells without any evidence of appressorial formation nor direct penetration. At 96 hai, fungal hyphae grew inter‐ and intracellularly in the lacunous parenchyma and also through tracheary elements. Pycnidia were first observed on the leaf surface at 96 hai. At 144 hai, conidia of P . longisetula were first liberated from the pycnidia. This study adds new information to better understand of the infection process of P . longisetula that may help in developing more effective disease control strategies.