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Detection of Putative Recombination Events in the 16 S Ribosomal RNA Gene of Some Members of Candidatus phytoplasma
Author(s) -
ElSayed Abdelaleim I.,
Boulila Moncef
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12218
Subject(s) - biology , phytoplasma , aster yellows , recombination , 16s ribosomal rna , gene , genetics , ribosomal rna , rna , polymerase chain reaction , restriction fragment length polymorphism
Recombination plays a major evolutionary role by creating genetic diversity and provides the potential to find rapid adaptation to new environmental conditions. We sought the occurrence of possible recombination events in the 16S ribosomal RNA gene of 60 accessions belonging to the group 16SrI of C andidatus phytoplasma (aster yellows phytoplasma). Three bioinformatic programs were used ( TOPALI v2.5, RECCO and RDP package). All the three programs indicated the presence of putative recombination signals in aligned sequences. Recombination events located in the 16 S ribosomal RNA gene revealed the presence of four recombining accessions gathering sugarcane grassy shoot phytoplasmas ( JF 928001, DQ 459439, EF 614269 and JN 223446).