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Analysis of Coat Protein of Peanut stunt virus Subgroup II Isolates from Alfalfa Fields in West Iran
Author(s) -
Pourrahim Reza,
Farzadfar Shirin
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12215
Subject(s) - biology , phylogenetic tree , genetic diversity , population , gene , genetics , nucleotide diversity , virology , genotype , haplotype , veterinary medicine , demography , sociology , medicine
Abstract Alfalfa fields in three western provinces of Iran were surveyed for Peanut stunt virus ( PSV ) during 2011 and 2012. Forty‐seven of 115 samples tested (41%) were infected with PSV . Phylogenetic analysis using coat protein ( CP ) gene sequences showed that the Iranian isolates belong to the subgroup II of PSV . Pairwise identity analysis revealed four groups representing four phylogenetic subgroups. PSV strains in subgroups III and IV are closely related to each other, as supported by the lowest nucleotide diversity, high pairwise nucleotide identity and high haplotype diversity as evidence of a recent population expansion after a genetic bottleneck. Using the maximum likelihood method, amino acid 86S in the CP gene of the Iranian PSV isolates was found to be under positive selection, although the likelihood ratio test statistics is not significant. This is the first report of the occurrence and phylogenetic relationships of Iranian PSV isolates in west Iran.