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Genetic Diversity and Pathogenic Variability of C olletotrichum truncatum Causing Anthracnose of Pepper in M alaysia
Author(s) -
Mahmodi Farshid,
Kadir Jugah,
Puteh Adam
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12213
Subject(s) - biology , genetic diversity , pepper , upgma , phylogenetic tree , veterinary medicine , botany , inoculation , genetic variation , genbank , horticulture , genetics , gene , population , medicine , demography , sociology
C olletotrichum truncatum was initially described from pepper and has been reported to infect 180 host genera in 55 plant families worldwide. Samples were collected from pepper plants showing typical anthracnose symptoms. Diseased samples after isolation were identified as C . truncatum based on morphological characters and ITS‐ rDNA and β ‐tubulin sequence data. Intersimple sequence repeat (ISSR) markers were used to estimate genetic diversity in C . truncatum from Malaysia. A set of 3 ISSR primers revealed a total 26 allele from the amplified products. Cluster analysis with UPGMA method clustered C . truncatum isolates into two main groups, which differed with a distance of 0.64. However, the genetic diversity of C . truncatum isolates showed correlation between genetic and geographical distribution, but it failed to reveal a relationship between clustering and pathogenic variability. Phylogenetic analyses discriminated the C . truncatum isolates from other reference C olletotrichum species derived from GenBank. Among the morphological characters, shape, colour of colony and growth rate in culture were partially correlated with the ISSR and phylogenetic grouping. Pathogenicity tests revealed that C . truncatum isolates were causal agents for pepper anthracnose. In the cross‐inoculation assays, C . truncatum isolates were able to produce anthracnose symptoms on tomato, eggplant, onion, lettuce and cabbage. A pathogenicity and cross‐inoculation studies indicated the potential of C . truncatum for virulence and dominancy on plant resistance.