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Detection and Molecular Variability of T urnip mosaic virus ( T u MV ) in S haanxi, C hina
Author(s) -
Zhao Lei,
Feng Chaohong,
Hao Xingan,
Wang Renrui,
Hu Lingyun,
Wang Qiaochun,
Wu Yunfeng
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12207
Subject(s) - biology , gene , genetic diversity , virus , virology , genetics , microbiology and biotechnology , population , demography , sociology
Turnip mosaic virus ( T u MV ) is one of the most devastating threats to oilseed rape by causing serious crop losses. A total of 86 leaf samples of oilseed rape from eight different locations in S haanxi, C hina, were tested by RT ‐ PCR for T u MV ; the results revealed an infection level of 43% by T u MV . The complete coat protein ( CP ) gene of 32 T u MV isolates was cloned and sequenced. Analysis of the CP gene with sequences from the database allowed the genetic classification of 170 T u MV isolates or sequences. Four genetic clusters were obtained: MB (mostly B rassica isolates), MR (mostly R adish isolates), IBR (mostly I ntermediate between B rassica and R adish clusters) and OBR (mostly outside B rassica and R adish clusters). All subgroups were slightly related to the hosts, but unrelated to geographical origins. Most of S haanxi T u MV isolates were on separate branches, compared with the 138 known isolates originating from other parts of the world. Our results help provide a better understanding of the genetic diversity of T u MV isolates infecting oilseed rape in S haanxi, C hina.