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The Complete Nucleotide Sequence of the RNA 1 of a C hinese Isolate of T omato chlorosis virus
Author(s) -
Karwitha Miriam,
Feng Zhike,
Yao Min,
Chen Xiaojiao,
Zhang Wenna,
Liu Xiaofan,
Tao Xiarong
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12203
Subject(s) - biology , chlorosis , virology , virus , plant virus , phylogenetic tree , sequence analysis , microbiology and biotechnology , nucleotide , horticulture , genetics , gene
Interveinal leaf chlorosis, brittleness, limited necrotic flecking or bronzing developed on greenhouse‐grown tobacco and tomato plants at N anjing A gricultural U niversity from 2010 to 2013. A positive RT ‐ PCR using a pair of degenerate primers for C rinivirus confirmed the diseased plants were infected with T omato chlorosis virus ( T o CV ). The complete RNA 1 genomic sequence of this T o CV isolate was determined; it comprises of 8596 nucleotides with four open reading frames. Phylogenetic analysis of T o CV isolates from diverse geographical regions categorized the T o CV isolates into two main groups. Group one consisted of C hinese, A merican‐ F lorida, G reek and B razilian isolates, while G roup two contained only the S panish isolate. The first group had two subgroups, one of C hinese and A merican‐ F lorida isolates, while the other subgroup had G reek and B razilian isolates. This is the first study of the complete nucleotide sequence of the RNA 1 of To CV isolated from C hina.