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Current Distribution of Huanglongbing (citrus greening disease) in India as Diagnosed by Real‐Time PCR
Author(s) -
Das Ashis K.,
Nerkar Sagar,
Bawage Swapnil,
Kumar Ashok
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12195
Subject(s) - biology , orange (colour) , greening , horticulture , primer (cosmetics) , botany , ecology , chemistry , organic chemistry
Abstract The widespread occurrence of Huanglongbing ( HLB ) was recorded in sixteen citrus growing states of India using the real‐time quantitative PCR and the derived threshold cycle ( C t) value. All the commercially important citrus varieties of mandarin, sweet orange, lime and lemon, pummelo and S atkara were infected with ‘ C andidatus Liberibacter asiaticus’ , the bacterium associated with HLB . C t values positive for HLB were found in all the states except A runachal P radesh. The primer–probe combination HLB as‐ HLB r‐ HLB p was found specific to C a . L . asiaticus and do not exhibit any cross‐reactivity with other pathogenic residents of citrus.