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First Report of P seudomonas lurida Causing Bacterial Leaf Spot on M iscanthus sinensis
Author(s) -
Lee Dong Wan,
Hong JinSung,
Kim SunHyung,
Kim Jin Won,
Kim Beom Seok
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12176
Subject(s) - biology , leaf spot , perennial plant , spots , proteobacteria , botany , bacteria , strain (injury) , inoculation , phylogenetic tree , 16s ribosomal rna , horticulture , gene , genetics , anatomy
M iscanthus spp. are large perennial wetland grasses that are receiving considerable attention as bioenergy crops. In late summer 2011, leaf spot symptoms were observed in a field of M iscanthus sinensis in Jeongseon, G angwon province, K orea. Bacterial strains that belonged to the γ‐Proteobacteria genus P seudomonas were isolated from the affected leaves. By phylogenetic analysis and phenotypic characterization, the representative strain MDM ‐03 was identified as P seudomonas lurida . Healthy M . sinensis leaves inoculated with MDM ‐03 developed leaf spots similar to those observed in field. Bacteria re‐isolated from the leaf lesions were identical to the original strain MDM ‐03 based on their cultural characteristics and 16S rDNA sequencing. This is the first report of bacterial leaf spot in M iscanthus sinensis .