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Identification of ‘ C andidatus P hytoplasma asteris' (16SrI‐B) C ausing Witches' Broom Disease of M allotus japonicus in K orea
Author(s) -
Han SangSub,
Oh ByungTaek,
KamalaKannan Seralathan
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12167
Subject(s) - phytoplasma , biology , broom , 16s ribosomal rna , phylogenetic tree , botany , operon , gene , genetics , polymerase chain reaction , restriction fragment length polymorphism , ecology , escherichia coli
Mallotus japonicus with witches' broom disease were observed in J eollabuk‐do, K orea. A phytoplasma from the infected leaves was identified, based on the 16S rDNA , 16S‐23S intergenic spacer region, and fragment of rp operon and tuf gene sequences. The 16S rDNA sequences exhibited maximum (99.7%) similarity with Iranian lettuce phytoplasma, the rp operon sequences exhibited 100% similarity with G oldenrain stunt phytoplasma, and the tuf gene sequences exhibited 99.8% similarity with Japanese spurge yellows phytoplasma. Results of the sequence analysis and phylogenetic studies confirmed that the phytoplasma associated with M . japonicus in K orea was an isolate of A ster Y ellows group (subgroup16SrI‐B).