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A Comparative and Phylogenetic Study of the D itylenchus dipsaci , D itylenchus destructor and D itylenchus gigas Populations Occurring in P oland
Author(s) -
Jeszke Arnika,
Budziszewska Marta,
Dobosz Renata,
Stachowiak Anna,
Protasewicz Dorota,
Wieczorek Przemyslaw,
ObrępalskaStęplowska Aleksandra
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12161
Subject(s) - biology , nematode , phylogenetic tree , population , botany , ecology , genetics , gene , demography , sociology
The genus D itylenchus contains more than 80 recognized nematode species with a very wide host range. The most serious species are D itylenchus dipsaci and D itylenchus destructor . Populations of D . dipsaci species complex were collected from A llium cepa , C ichorium endivia and P hlox paniculata in P oland. The D itylenchus gigas population was collected from V icia faba minor , and populations of D . destructor , from S olanum tuberosum spp. tuberosum . Analyses of the r DNA sequences spanning both ITS 1 and ITS 2 fragment regions were carried out on the collected populations. The obtained DNA sequences were compared with those DNA sequences deposited in G en B ank of populations isolated in other countries. Phylogenetic analysis was performed using the data obtained from the DNA sequence comparisons. The results indicated that there is no clear distinction between E uropean and non‐ E uropean populations within D . dipsaci . The results also showed no clear distinction between populations isolated from different host plant species, including populations found in P oland. The populations of D . destructor described here constitute a common group together with A merican and C hinese populations belonging to the haplotype C of the D . destructor species. On the other hand, the D . gigas population was localized separately from those populations that have been described up until now, from E urope and A frica. This is also the first report on the occurrence of D . gigas in P oland.