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Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty
Author(s) -
Dal Zotto Angélica,
Balzarini Mónica,
Raigón Juan M.,
Rossini Mirta N.,
Ducasse Daniel A.
Publication year - 2014
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12160
Subject(s) - biology , nicotiana benthamiana , pox virus , virology , prunus salicina , serology , phylogenetic tree , capsid , genome , prunus , potyvirus , strain (injury) , plant virus , virus , genetics , botany , gene , antibody , anatomy
A Plum pox virus ( PPV ) isolate detected in a Japanese plum orchard in Pocito (San Juan, Argentina) was transmitted mechanically to Prunus tomentosa and Nicotiana benthamiana . DAS ‐ ELISA and DASI ‐ ELISA indicated the virus presence and serological relationship with D‐strain isolates; IC ‐ RT ‐ PCR amplified a 1.2‐kb fragment of the virus genome encoding the CP ‐3′ nc region. The analysis of the sequence showed the presence of the DAG motif at the 5′ end of the capsid protein and the Rsa I and Alu I sites at the 3′ end. The phylogenetic relationships and multiple alignment with PPV isolates from NCBI database indicated greatest (+98%) homology with the D strain and close identity with MNAT 1 ( AF360579 ) USA peach isolate. The sequence analysed showed two amino acid mutations towards the 5′ N‐terminus of CP (the most variable region) with respect to a consensus of PPV D‐strain isolates. This is the first molecular characterization of 3′terminal genome region of PPV isolate to confirm D strain in a Japanese plum from Argentina.