Natural Infection of  M aize by   C ucumber  M osaic  V irus  in  C hina | Zendy

Wang Rong | Zendy; Wang Nian | Zendy; Ye Ting | Zendy; Chen Hui | Zendy; Fan Zaifeng | Zendy; Zhou Tao | Zendy

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Natural Infection of M aize by C ucumber M osaic V irus in C hina

Author(s) -

Wang Rong,

Wang Nian,

Ye Ting,

Chen Hui,

Fan Zaifeng,

Zhou Tao

Publication year - 2013

Publication title -

journal of phytopathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.53

H-Index - 60

eISSN - 1439-0434

pISSN - 0931-1785

DOI - 10.1111/jph.12141

Subject(s) - biology , cucumber mosaic virus , microbiology and biotechnology , virology , blot , phylogenetic tree , messenger rna , virus , plant virus , gene , biochemistry

In J uly 2012, symptoms of irregular mosaic stripe and mottle were observed on maize leaves in field in B eijing, C hina. The causal pathogen was identified to be C ucumber mosaic virus ( CMV ) based upon reverse transcription‐ PCR , enzyme‐linked immunosorbent assay, W estern blotting and fulfilment of K och's postulates. The isolate was named ZMBJ ‐ CMV . Full sequence of ZMBJ ‐ CMV RNA 3 was determined, and it had the highest identity to that of strain K ‐ CMV (95.03%) and SD ‐ CMV (94.96%). Phylogenetic analysis revealed ZMBJ ‐ CMV clustered with K ‐ CMV and SD ‐ CMV in subgroup IB . To our knowledge, this is the first report on the natural infection and phylogenetic analysis of CMV on maize in C hina.

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