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Natural Infection of M aize by C ucumber M osaic V irus in C hina
Author(s) -
Wang Rong,
Wang Nian,
Ye Ting,
Chen Hui,
Fan Zaifeng,
Zhou Tao
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12141
Subject(s) - biology , cucumber mosaic virus , microbiology and biotechnology , virology , blot , phylogenetic tree , messenger rna , virus , plant virus , gene , biochemistry
Abstract In J uly 2012, symptoms of irregular mosaic stripe and mottle were observed on maize leaves in field in B eijing, C hina. The causal pathogen was identified to be C ucumber mosaic virus ( CMV ) based upon reverse transcription‐ PCR , enzyme‐linked immunosorbent assay, W estern blotting and fulfilment of K och's postulates. The isolate was named ZMBJ ‐ CMV . Full sequence of ZMBJ ‐ CMV RNA 3 was determined, and it had the highest identity to that of strain K ‐ CMV (95.03%) and SD ‐ CMV (94.96%). Phylogenetic analysis revealed ZMBJ ‐ CMV clustered with K ‐ CMV and SD ‐ CMV in subgroup IB . To our knowledge, this is the first report on the natural infection and phylogenetic analysis of CMV on maize in C hina.