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Optimization of Culturing Conditions of a Strain of P hytophthora capsici Pathogenic to Habanero Pepper ( C apsicum chinense )
Author(s) -
FloresGiubi María Eugenia,
BritoArgáez Ligia,
GarcíaSosa Karlina,
EscalanteErosa Fabiola,
IslasFlores Ignacio,
PeñaRodríguez Luis Manuel
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12138
Subject(s) - pepper , capsicum chinense , biology , mycelium , oomycete , phytophthora capsici , colletotrichum capsici , wilting , aeration , phytotoxicity , horticulture , inoculation , botany , microbiology and biotechnology , fungicide , pathogen , ecology
P hytophthora capsici is an oomycete known as the causal agent of wilting disease in Capsicum spp., which causes rotting of roots, crowns, stems, leaves and fruits. To date, little is known about the production of phytotoxic metabolites by P . capsici or their role in the infection process. As part of a project directed towards the isolation and identification of phytotoxins produced by a strain of P . capsici pathogenic to habanero pepper ( C apsicum chinense ), we have evaluated the effect of factors such as aeration, light and culture medium on the production of mycelium and phytotoxic metabolites by P . capsici . The results showed that culturing P . capsici in potato dextrose broth ( PDB ) containing habanero pepper leaf infusion, in the dark and under still conditions, results in a high production of mycelium and a high phytotoxicity of the culture filtrate, in the shortest period of time.