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Molecular Detection and Identification of a 16 S rVI Group Phytoplasma Associated with Tomato Big Bud Disease in X injiang, C hina
Author(s) -
Du Yejuan,
Mou Haiqing,
Shi Baoping,
Xu Xia,
Xiang Benchun
Publication year - 2013
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12136
Subject(s) - phytoplasma , biology , restriction fragment length polymorphism , microbiology and biotechnology , genotype , genetics , polymerase chain reaction , gene
In 2010, tomato plants with big bud symptoms were observed in X injiang, C hina. PCR products of approximately 1.2 and 2.8 kb were amplified from infected tomato tissues but not from asymptomatic plants. A comparison of 16 S rDNA sequences showed that the casual tomato big bud ( TBB ) phytoplasma was closely (99%) related to the ‘ C andidatus P hytoplasma trifolii’ (16 S rVI group). The TBB phytoplasma clustered into one branch with the L oofah witches'‐broom phytoplasma according to the 23 S rDNA analysis but with no other member of the 16 S rVI group. The cause of TBB symptoms was identified as ‘ C a . P hytoplasma trifolii' (16 S rVI group) by PCR , virtual RFLP and sequencing analyses. This is the first report of a phytoplasma related to ‘ C a . P hytoplasma trifolii' causing TBB disease in C hina.