Molecular Detection and Identification of a 16 S rVI Group Phytoplasma Associated with Tomato Big Bud Disease in  X injiang,  C hina | Zendy

Du Yejuan | Zendy; Mou Haiqing | Zendy; Shi Baoping | Zendy; Xu Xia | Zendy; Xiang Benchun | Zendy

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Molecular Detection and Identification of a 16 S rVI Group Phytoplasma Associated with Tomato Big Bud Disease in X injiang, C hina

Author(s) -

Du Yejuan,

Mou Haiqing,

Shi Baoping,

Xu Xia,

Xiang Benchun

Publication year - 2013

Publication title -

journal of phytopathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.53

H-Index - 60

eISSN - 1439-0434

pISSN - 0931-1785

DOI - 10.1111/jph.12136

Subject(s) - phytoplasma , biology , restriction fragment length polymorphism , microbiology and biotechnology , genotype , genetics , polymerase chain reaction , gene

In 2010, tomato plants with big bud symptoms were observed in X injiang, C hina. PCR products of approximately 1.2 and 2.8 kb were amplified from infected tomato tissues but not from asymptomatic plants. A comparison of 16 S rDNA sequences showed that the casual tomato big bud ( TBB ) phytoplasma was closely (99%) related to the ‘ C andidatus P hytoplasma trifolii’ (16 S rVI group). The TBB phytoplasma clustered into one branch with the L oofah witches'‐broom phytoplasma according to the 23 S rDNA analysis but with no other member of the 16 S rVI group. The cause of TBB symptoms was identified as ‘ C a . P hytoplasma trifolii' (16 S rVI group) by PCR , virtual RFLP and sequencing analyses. This is the first report of a phytoplasma related to ‘ C a . P hytoplasma trifolii' causing TBB disease in C hina.

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